4TLS
Crystal Structure of Human Transthyretin Glu92Pro Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 42.380, 86.080, 64.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.360 - 1.350 |
R-factor | 0.1857 |
Rwork | 0.185 |
R-free | 0.19210 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data scaling software | XSCALE |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 64.360 |
High resolution limit [Å] | 1.350 |
Number of reflections | 52487 |
<I/σ(I)> | 14.15 |
Completeness [%] | 99.9 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% (W/V) PEG-1000, 0.1M Imidazole, 0.2M Calcium Acetate |