4TL7

Crystal structure of N-terminal C1 domain of KaiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-27
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 31 2 1
Unit cell lengths	108.165, 108.165, 224.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.853 - 1.936
R-factor	0.1519
Rwork	0.150
R-free	0.19140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.719
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.936
Number of reflections	110530
<I/σ(I)>	48.1
Completeness [%]	97.1
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	PEG 400 or PEG 8000