4TKP

Complex of Ubc13 with the RING domain of the TRIM5alpha retroviral restriction factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-02
Detector	PSI PILATUS 6M
Wavelength(s)	1.2823
Spacegroup name	P 41 21 2
Unit cell lengths	78.170, 78.170, 82.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.930 - 2.080
R-factor	0.2257
Rwork	0.224
R-free	0.25530
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.664
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.930	2.190
High resolution limit [Å]	2.080	2.080
Rmerge		0.588
Number of reflections	15907
<I/σ(I)>	23.9	2.4
Completeness [%]	99.7	100
Redundancy	7	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	2.0 M ammonium sulfate, 0.1 M bis-tris