4TKP
Complex of Ubc13 with the RING domain of the TRIM5alpha retroviral restriction factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.2823 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 78.170, 78.170, 82.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.930 - 2.080 |
R-factor | 0.2257 |
Rwork | 0.224 |
R-free | 0.25530 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.664 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.930 | 2.190 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.588 | |
Number of reflections | 15907 | |
<I/σ(I)> | 23.9 | 2.4 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 2.0 M ammonium sulfate, 0.1 M bis-tris |