4TKN
Structure of the SNX17 FERM domain bound to the second NPxF motif of KRIT1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97926 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.924, 191.202, 46.593 |
Unit cell angles | 90.00, 110.25, 90.00 |
Refinement procedure
Resolution | 47.880 - 3.000 |
R-factor | 0.223 |
Rwork | 0.218 |
R-free | 0.26810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4GXB chain A |
RMSD bond length | 0.005 |
RMSD bond angle | 1.245 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.120 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.241 | |
Number of reflections | 18042 | |
<I/σ(I)> | 10.8 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.9 M Ammonium Sulfate, 0.1 M HEPES pH 7.5, 5% PEG 400 |