4S2C
Covalent complex of E. coli transaldolase TalB with fructose-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91914 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.656, 91.152, 130.725 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.430 - 2.200 |
R-factor | 0.15586 |
Rwork | 0.153 |
R-free | 0.21103 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ucw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.311 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.430 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.094 | 0.491 |
Number of reflections | 41815 | |
<I/σ(I)> | 12.7 | 3.7 |
Completeness [%] | 99.5 | 97.6 |
Redundancy | 4.8 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.2 | 0.2 M DL-malic acid, pH 7.0, 19% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |