4S22
Crystal structure of K29 linked di-Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.450, 69.245, 60.055 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 60.050 - 2.300 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.926 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.050 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.096 | 0.440 |
| Number of reflections | 11542 | |
| <I/σ(I)> | 8.71 | |
| Completeness [%] | 94.1 | 92.9 |
| Redundancy | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 285 | 100mM MES, 200mM potassium iodide and 25% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
