4S0W

Wild type T4 lysozyme structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-22
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54187
Spacegroup name	P 31
Unit cell lengths	52.630, 52.630, 99.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.601 - 2.117
R-factor	0.1718
Rwork	0.170
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	206l
RMSD bond length	0.003
RMSD bond angle	0.559
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1683))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.579
High resolution limit [Å]	2.117
Rmerge	0.104
Number of reflections	17563
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH UNDER OIL	7.5	296	0.5 M ammonium sulfate, 0.1 M HEPES, 30% MPD, pH 7.5, BATCH UNDER OIL, temperature 296K