4RYY
Crystal structure of RPE65 in complex with R-emixustat and palmitate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97917 |
Spacegroup name | P 65 |
Unit cell lengths | 175.844, 175.844, 86.424 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.910 - 2.300 |
R-factor | 0.17937 |
Rwork | 0.177 |
R-free | 0.21533 |
Structure solution method | rigid body refinement |
Starting model (for MR) | 3fsn |
RMSD bond length | 0.012 |
RMSD bond angle | 1.543 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.120 | 0.922 |
Number of reflections | 67069 | |
<I/σ(I)> | 8.8 | 1.22 |
Completeness [%] | 99.4 | 98.2 |
Redundancy | 3.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 281 | 40% PEG 300, 200 mM NaCl, 100 mM CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |