4RXH
Crystal Structure of Importin-alpha from Neurospora crassa complexed with SV40NLS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.127, 64.494, 185.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.975 - 1.755 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ul1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.098 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.980 | 1.820 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.843 | |
Number of reflections | 54993 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 99.8 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 20 Mm Bicine pH 8.5, 20%(w/v) PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 297K |