4RWI
Crystal structure of V561M FGFR1 gatekeeper mutation (C488A, C584S, V561M), apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-06-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 211.061, 50.550, 66.529 |
Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
Resolution | 47.499 - 2.292 |
R-factor | 0.188 |
Rwork | 0.185 |
R-free | 0.23620 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3ky2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.133 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
High resolution limit [Å] | 2.290 | 6.210 | 2.290 |
Rmerge | 0.030 | 0.177 | |
Number of reflections | 30355 | ||
<I/σ(I)> | 36.3 | ||
Completeness [%] | 99.7 | 99.3 | 96 |
Redundancy | 3.7 | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 0.1 M sodium cacodylate pH 6.4, 30% PEG 8000, 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |