4RUG
Cyrstal structure of SLIT-ROBO Rho GTPase-activating protein 2 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.510, 31.350, 62.830 |
| Unit cell angles | 90.00, 113.07, 90.00 |
Refinement procedure
| Resolution | 57.810 - 1.730 |
| R-factor | 0.19054 |
| Rwork | 0.189 |
| R-free | 0.22216 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.609 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.810 | 1.840 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.118 | |
| Number of reflections | 18107 | |
| <I/σ(I)> | 9.09 | |
| Completeness [%] | 95.0 | 0.89 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.25 | 277 | 2.35M Ammonium Sulphate; 0.1M MES, pH 6.25, VAPOR DIFFUSION, temperature 277K |
