4RU2
Crystal structure of a RNA-binding protein 39 (RBM39) in complex with fragment of splicing factor (U2AF) from Mus musculus at 2.20 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-15 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 32 |
Unit cell lengths | 127.283, 127.283, 78.864 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.432 - 2.200 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s6e |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.432 | 2.260 | |
High resolution limit [Å] | 2.200 | 9.830 | 2.200 |
Rmerge | 0.116 | 0.036 | 0.830 |
Number of reflections | 72455 | 793 | 5269 |
<I/σ(I)> | 9.71 | 26.6 | 2 |
Completeness [%] | 99.7 | 98.3 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M potassium chloride, 15.0% polyethylene glycol monomethyl ether 5000, 0.1M HEPES pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |