4RU2

Crystal structure of a RNA-binding protein 39 (RBM39) in complex with fragment of splicing factor (U2AF) from Mus musculus at 2.20 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-15
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 32
Unit cell lengths	127.283, 127.283, 78.864
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.432 - 2.200
R-factor	0.176
Rwork	0.174
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s6e
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.432		2.260
High resolution limit [Å]	2.200	9.830	2.200
Rmerge	0.116	0.036	0.830
Number of reflections	72455	793	5269
<I/σ(I)>	9.71	26.6	2
Completeness [%]	99.7	98.3	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M potassium chloride, 15.0% polyethylene glycol monomethyl ether 5000, 0.1M HEPES pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K