4RTA

Cystal structure of the Dpy30 for MLL/SET1 COMPASS H3K4 trimethylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9998
Spacegroup name	P 41 21 2
Unit cell lengths	89.579, 89.579, 53.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.214 - 2.123
R-factor	0.2081
Rwork	0.207
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g36
RMSD bond length	0.007
RMSD bond angle	1.027
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.210
High resolution limit [Å]	2.123	2.123
Rmerge	0.100	0.630
Number of reflections	12673
<I/σ(I)>	24.8	5
Completeness [%]	99.6	100
Redundancy	10	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.9	291	0.1M COCADYLATE SODIUM, SODIUM FORMATE 4.2M, pH 5.9, EVAPORATION, temperature 291K