4RSV

Human Obscurin Ig58 Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	170
Detector technology	CCD
Collection date	2014-04-10
Detector	ADSC QUANTUM 315r
Spacegroup name	P 61 2 2
Unit cell lengths	44.482, 44.482, 177.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.640 - 2.410
R-factor	0.26
Rwork	0.260
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cr6 2yz8 2e7b 2rq8
RMSD bond length	0.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.640	2.620
High resolution limit [Å]	2.411	2.560
Rmerge	0.033	0.317
Number of reflections	4329
<I/σ(I)>	29.47	3.08
Completeness [%]	95.8	69.2
Redundancy	3.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	298	0.3-0.5M NaCl 19-23% PEG 3350 10.52-11.35 mg/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K