4RSS
Crystal structure of tyrosine-protein kinase SYK with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.964, 88.281, 40.850 |
| Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
| Resolution | 44.140 - 1.830 |
| R-factor | 0.21375 |
| Rwork | 0.212 |
| R-free | 0.24869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 24171 | |
| Completeness [%] | 97.0 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 10-20% (v/v) PEG 3350, 100mM Tris-HCl (pH 8.5) , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
