4RSE
Crystal structure of RPE65 in complex with MB-001 and palmitate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0750 |
Spacegroup name | P 65 |
Unit cell lengths | 175.639, 175.639, 86.611 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.900 - 2.390 |
R-factor | 0.19784 |
Rwork | 0.196 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fsn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.398 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.390 |
Number of reflections | 59880 |
Completeness [%] | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 281 | 40% PEG 300 200 mM NaCl 100 mM CHES-NAOH, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |