4RS4
Crystal structure and mutational analysis of the endoribonuclease from human coronavirus 229E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-09 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 127.931, 143.168, 174.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.955 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gth |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.895 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.250 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.950 |
| Number of reflections | 61287 | |
| Completeness [%] | 98.7 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M HEPES pH 7.5, 150mM NaCl, 10% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
