4RS1

Crystal structure of receptor-cytokine complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-15
Detector	ADSC QUANTUM 315r
Spacegroup name	P 2 21 21
Unit cell lengths	49.801, 77.169, 117.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.020 - 2.680
R-factor	0.228
Rwork	0.225
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.409
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.805	2.780
High resolution limit [Å]	2.680	2.680
Rmerge	0.060
Number of reflections	13058
<I/σ(I)>	19.8
Completeness [%]	98.1	98.1
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	22% PEG8000, 1% MPD, 5MM DITHIOTHREITOL, 100MM IMIDAZOLE, PH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K