4RQN

Crystal structure of the native BICC1 SAM Domain R924E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	41.680, 59.220, 68.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.844 - 2.000
R-factor	0.2175
Rwork	0.212
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rqm
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.220	59.220	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.092	0.080	0.486
Number of reflections	11813
<I/σ(I)>	14.94	24.28	4.38
Completeness [%]	98.5	98.1	98.7
Redundancy	12.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	30% PEG-550 MME, 100mM MES, 5mM zinc sulfate, pH 6.5, vapor diffusion, hanging drop, temperature 298K