4RQF

human Seryl-tRNA synthetase dimer complexed with one molecule of tRNAsec

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99
Spacegroup name	P 21 21 2
Unit cell lengths	160.377, 108.894, 89.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.625 - 3.503
R-factor	0.256
Rwork	0.253
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l87 3a3a
RMSD bond length	0.010
RMSD bond angle	1.238
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.680
High resolution limit [Å]	3.503	3.500
Rmerge	0.150	0.956
Number of reflections	19748
<I/σ(I)>	12.4
Completeness [%]	98.7	100
Redundancy	5.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	18%(m/v) PEG3350, 0.1M NaCl, 0.1M Tris-HCl (pH8.0), 0.1M Sodium malonate pH7.0., VAPOR DIFFUSION, temperature 298K