4RP6
Structure of the amyloid-forming segment LTIITLE from p53 (residues 252-258)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 4.811, 12.599, 21.340 |
| Unit cell angles | 86.59, 89.29, 79.15 |
Refinement procedure
| Resolution | 21.302 - 1.703 |
| R-factor | 0.1655 |
| Rwork | 0.163 |
| R-free | 0.19190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.302 | 1.780 |
| High resolution limit [Å] | 1.703 | 1.720 |
| Rmerge | 0.096 | 0.505 |
| Number of reflections | 512 | |
| <I/σ(I)> | 10.6 | 11.6 |
| Completeness [%] | 97.0 | 91.4 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | reservoir contained 0.1 M Tris buffer pH 8.5 and 20% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
