4RP5
Crystal Structure of the L27 domain of Discs Large 1 (target ID NYSGRC-010766) from Drosophila melanogaster (space group P21)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-03-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.546, 47.818, 64.424 |
Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
Resolution | 27.016 - 1.650 |
R-factor | 0.1835 |
Rwork | 0.182 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rp3 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.680 |
High resolution limit [Å] | 1.650 | 4.470 | 1.650 |
Rmerge | 0.047 | 0.031 | 0.544 |
Number of reflections | 20902 | ||
<I/σ(I)> | 14.2 | ||
Completeness [%] | 99.7 | 96.8 | 99.8 |
Redundancy | 4.1 | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 0.2 M Sodium Format, 20 % PEG 3350, 3 % 1,5-Diaminopentane dihydrochloride, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |