4RNK
Sequence and structure of a self-assembled 3-D DNA crystal: D(GGAAAATTTGGAG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979200 |
Spacegroup name | P 64 |
Unit cell lengths | 39.729, 39.729, 55.989 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.410 - 2.080 |
R-factor | 0.20444 |
Rwork | 0.201 |
R-free | 0.23930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p1y |
RMSD bond length | 0.012 |
RMSD bond angle | 1.800 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.990 | 2.190 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.051 | 0.390 |
Number of reflections | 3045 | |
<I/σ(I)> | 28.4 | 3.5 |
Redundancy | 5.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 120mM Magnesium Formate 50mM Lithium Chloride 10% MPD, pH none, EVAPORATION, temperature 298K |