4RMW
Crystal structure of the D76A Beta-2 Microglobulin mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-13 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.87 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.624, 28.992, 56.293 |
Unit cell angles | 90.00, 127.20, 90.00 |
Refinement procedure
Resolution | 38.593 - 1.400 |
R-factor | 0.1829 |
Rwork | 0.180 |
R-free | 0.21320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yxf |
RMSD bond length | 0.020 |
RMSD bond angle | 1.948 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.840 | 1.460 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.066 | 0.482 |
Number of reflections | 19921 | |
<I/σ(I)> | 11.7 | 2.5 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 21% PEG4000, 15% glycerol, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |