4RMW
Crystal structure of the D76A Beta-2 Microglobulin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.87 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.624, 28.992, 56.293 |
| Unit cell angles | 90.00, 127.20, 90.00 |
Refinement procedure
| Resolution | 38.593 - 1.400 |
| R-factor | 0.1829 |
| Rwork | 0.180 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxf |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.948 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.840 | 1.460 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.066 | 0.482 |
| Number of reflections | 19921 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 21% PEG4000, 15% glycerol, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
