4RM8
Crystal structure of human ezrin in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.007, 111.808, 68.384 |
| Unit cell angles | 90.00, 113.01, 90.00 |
Refinement procedure
| Resolution | 39.403 - 1.900 |
| R-factor | 0.1826 |
| Rwork | 0.181 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i1j |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.894 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.850 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.112 | 0.342 |
| Number of reflections | 67737 | |
| <I/σ(I)> | 7.1 | 2 |
| Completeness [%] | 95.9 | 92 |
| Redundancy | 3.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | 0.1M Bis-Tris pH 5.9, 15% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
