4RJD
TFP bound in alternate orientations to calcium-saturated Calmodulin C-Domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-14 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.606, 85.637, 35.341 |
| Unit cell angles | 90.00, 93.00, 90.00 |
Refinement procedure
| Resolution | 27.230 - 2.000 |
| R-factor | 0.1809 |
| Rwork | 0.177 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lin |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.452 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.230 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.029 | 0.118 |
| Number of reflections | 8955 | |
| <I/σ(I)> | 24 | 8.1 |
| Completeness [%] | 90.5 | 94.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 288 | 50 mM HEPES, 100 mM KCl, 1mM MgCl2, 5 mM NTA, 50 uM EGTA, with 500 ul of 200 mM potassium thiocyanate, 20% polyethylene glycol 3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
