4RID
Human FAN1 nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 146.882, 156.885, 205.396 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.2471 |
| Rwork | 0.246 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.335 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.000 | 70.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 7.110 | 3.300 |
| Rmerge | 0.090 | 0.031 | 0.714 |
| Number of reflections | 68520 | ||
| <I/σ(I)> | 3.8 | ||
| Completeness [%] | 99.4 | 95.7 | 99.7 |
| Redundancy | 6.9 | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 3350, NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
