4RID
Human FAN1 nuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97925 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 146.882, 156.885, 205.396 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.300 |
R-factor | 0.2471 |
Rwork | 0.246 |
R-free | 0.28800 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.335 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 70.000 | 70.000 | 3.420 |
High resolution limit [Å] | 3.300 | 7.110 | 3.300 |
Rmerge | 0.090 | 0.031 | 0.714 |
Number of reflections | 68520 | ||
<I/σ(I)> | 3.8 | ||
Completeness [%] | 99.4 | 95.7 | 99.7 |
Redundancy | 6.9 | 6.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 3350, NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |