4RH3
AMPPCP-bound structure of human platelet phosphofructokinase in an R-state, crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 132.911, 132.911, 397.168 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.900 - 3.020 |
| R-factor | 0.14834 |
| Rwork | 0.146 |
| R-free | 0.18730 |
| Structure solution method | 4WLO |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.494 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 8.920 | 3.200 |
| High resolution limit [Å] | 3.020 | 6.360 | 3.020 |
| Rmerge | 0.184 | 0.011 | 0.011 |
| Number of reflections | 80705 | ||
| <I/σ(I)> | 12.32 | 30.36 | 2.02 |
| Completeness [%] | 99.9 | 100 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 0,1 M MES pH 6.0 23% v/v MPD, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
