4REC
A nuclease-DNA complex form 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.910, 109.600, 115.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.600 - 2.200 |
R-factor | 0.2167 |
Rwork | 0.215 |
R-free | 0.25410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | an initial model phased by a low-resolution iodine-anomalous dataset |
RMSD bond length | 0.009 |
RMSD bond angle | 1.152 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.600 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | |
Number of reflections | 89740 | |
<I/σ(I)> | 9 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | micro-batch under oil | 300 | 0.1M potassium iodide, 0.1mM spermidine, 18% PEG3350, 0.1M BisTris propane, micro-batch under oil, temperature 300K |