4RDU

Crystal structure of a distal-less homeobox protein 5 (Dlx5) from Homo sapiens at 1.85 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-24
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 1
Unit cell lengths	34.182, 42.913, 56.104
Unit cell angles	84.13, 77.33, 67.13

Refinement procedure

Resolution	22.303 - 1.850
R-factor	0.185
Rwork	0.183
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ig7
RMSD bond length	0.011
RMSD bond angle	1.316
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.303	22.303	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge		0.047	0.271
Total number of observations		322	3315
Number of reflections	23348
<I/σ(I)>	8	14.4	2.5
Completeness [%]	95.5	66.1	94.1
Redundancy	1.9	1.8	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	0.08M sodium chloride, 45.00% 2-methyl-2,4-pentanediol, 0.012M spermine tetrahydrochloride, 0.1M sodium cacodylate pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 289K