4RD2
Structure of aIF2-gamma H97A variant from Sulfolobus solfataricus bound to GDPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.300, 60.470, 144.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.344 - 1.585 |
R-factor | 0.1478 |
Rwork | 0.145 |
R-free | 0.20220 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4rd1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.039 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.8.4_1496) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.340 | 1.680 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.080 | 0.816 |
Number of reflections | 55647 | |
<I/σ(I)> | 13.3 | 1.97 |
Completeness [%] | 99.4 | 97.2 |
Redundancy | 7.16 | 7.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 60%MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |