4RD0

Structure of aIF2-gamma D19A variant from Sulfolobus solfataricus bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.979180
Spacegroup name	P 21 21 21
Unit cell lengths	45.890, 59.870, 144.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.060 - 1.713
R-factor	0.1484
Rwork	0.145
R-free	0.20300
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4rcz
RMSD bond length	0.007
RMSD bond angle	1.082
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.060	1.820
High resolution limit [Å]	1.710	1.710
Rmerge	0.144	0.617
Number of reflections	43359
<I/σ(I)>	8.31	2.46
Completeness [%]	99.4	96.9
Redundancy	6.19	6.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	70% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K