4RCJ

Crystal structure of YTHDF1 YTH domain in complex with 5mer m6A RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791829
Spacegroup name	P 1 21 1
Unit cell lengths	37.648, 40.029, 59.308
Unit cell angles	90.00, 93.41, 90.00

Refinement procedure

Resolution	40.030 - 1.600
R-factor	0.1732
Rwork	0.171
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rci
RMSD bond length	0.019
RMSD bond angle	1.944
Data scaling software	Aimless (0.3.6)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.030	40.030	1.630
High resolution limit [Å]	1.600	8.790	1.600
Rmerge	0.102	0.035	0.609
Total number of observations		505	3203
Number of reflections	23138
<I/σ(I)>	9.4	21	1.8
Completeness [%]	99.4	97.5	93.5
Redundancy	3.6	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% PEG-3350, 0.2 M sodium chloride, 0.1 M bis-tris, pH 6.5, vapor diffusion, sitting drop, temperature 293K