4RAL
Crystal structure of insulin degrading enzyme in complex with macrophage inflammatory protein 1 beta
Replaces: 4QLDExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 65 |
| Unit cell lengths | 263.117, 263.117, 90.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.263 - 3.148 |
| R-factor | 0.1918 |
| Rwork | 0.191 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cww |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.848 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.263 | |
| High resolution limit [Å] | 3.148 | 3.148 |
| Rmerge | 0.209 | |
| Number of reflections | 59484 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 95.5 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 13% PEG5000 MME, 100 mM HEPES, pH 7.0, 10% Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
