4RAJ
Crystal structure of heme oxygenase 2 from Chlamydomonas reinhardtii without heme.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12709 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.930, 134.770, 80.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.865 - 1.840 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n45 4g7l 1wov 2rgz 1we1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.968 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.042 | 0.519 |
| Number of reflections | 20218 | |
| <I/σ(I)> | 17.45 | 2.5 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 18% PEG Monomethyl Ether 5000, 0.2M NaCl, 0.1M Bis Tris pH 6.5. , VAPOR DIFFUSION, SITTING DROP, temperature 294K |
