4RA0
An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.370, 112.370, 361.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.010 - 3.070 |
| R-factor | 0.20416 |
| Rwork | 0.202 |
| R-free | 0.24255 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c5d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.010 | 3.150 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Rmerge | 0.118 | 1.000 |
| Number of reflections | 50592 | |
| <I/σ(I)> | 20.4 | 2.11 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.4 M Li2SO4, 0.1 M Tris.HCl(pH 8.5), 5% Glycerol, 2mM NiSO4 , VAPOR DIFFUSION, HANGING DROP, temperature 295K |
