4R9Y
Crystal structure of KKOFab in complex with platelet factor 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.490, 99.340, 261.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.110 |
| R-factor | 0.31985 |
| Rwork | 0.317 |
| R-free | 0.38218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.620 | 4.170 |
| High resolution limit [Å] | 4.100 | 4.510 | 4.100 |
| Rmerge | 0.236 | 0.441 | |
| Number of reflections | 9343 | ||
| Completeness [%] | 87.9 | 77.1 | 61.3 |
| Redundancy | 6.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 289 | 7% PEG6000, 0.1 M Tris-HCl, pH7.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
