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4R96

Structure of a Llama Glama Fab 48A2 against human cMet

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSOLEIL BEAMLINE PROXIMA 1
Synchrotron siteSOLEIL
BeamlinePROXIMA 1
Temperature [K]100
Detector technologyPIXEL
Collection date2011-07-04
DetectorPSI PILATUS 6M
Spacegroup nameP 21 21 21
Unit cell lengths107.020, 121.540, 185.900
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution43.390 - 3.310
R-factor0.221
Rwork0.217
R-free0.26100
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2vxs
RMSD bond length0.009
RMSD bond angle1.250
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwareBUSTER (2.9.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]45.0003.400
High resolution limit [Å]3.3003.300
Rmerge0.1200.586
Number of reflections35909
<I/σ(I)>72.4
Completeness [%]97.597.1
Redundancy3.33.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
16277in 1.4 M Na Malonate pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K

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