4R96
Structure of a Llama Glama Fab 48A2 against human cMet
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-04 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.020, 121.540, 185.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.390 - 3.310 |
R-factor | 0.221 |
Rwork | 0.217 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vxs |
RMSD bond length | 0.009 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.120 | 0.586 |
Number of reflections | 35909 | |
<I/σ(I)> | 7 | 2.4 |
Completeness [%] | 97.5 | 97.1 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 277 | in 1.4 M Na Malonate pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |