4R90
Anti CD70 Llama glama Fab 27B3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.570, 66.880, 125.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.710 - 1.746 |
| R-factor | 0.16391 |
| Rwork | 0.163 |
| R-free | 0.18499 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vxs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 1.850 |
| High resolution limit [Å] | 1.746 | 1.746 |
| Rmerge | 0.074 | 0.600 |
| Number of reflections | 55037 | |
| <I/σ(I)> | 16.4 | 3 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 6.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 2M Ammonium sulphate (Sigma-Aldrich, A4418) and 0.15 M Na citrate pH 5.5 (Sigma-Aldrich, PHR1416). , VAPOR DIFFUSION, SITTING DROP, temperature 277K |
