4R8P

Crystal structure of the Ring1B/Bmi1/UbcH5c PRC1 ubiquitylation module bound to the nucleosome core particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	104.923, 180.049, 375.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 3.285
R-factor	0.1993
Rwork	0.197
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	polyalanine models of PDB entries 3RPG and 3LZ0
RMSD bond length	0.003
RMSD bond angle	0.504
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	3.460
High resolution limit [Å]	3.285	10.390	3.285
Rmerge		0.012	0.640
Total number of observations		5896	31034
Number of reflections	54142
<I/σ(I)>	16.5	53.8	1.2
Completeness [%]	99.4	91.3	99.8
Redundancy	3.9	3.5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Modified micro batch under oil	7.5	294	25 mM HEPES pH 7.5, 80 mM NH4NO3, 3 % PEG2000-MME, Modified micro batch under oil, temperature 294K