4R82
Streptomyces globisporus C-1027 NADH:FAD oxidoreductase SgcE6 in complex with NAD and FAD fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.046, 62.830, 70.089 |
| Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
| Resolution | 34.928 - 1.659 |
| R-factor | 0.1644 |
| Rwork | 0.163 |
| R-free | 0.18760 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.158 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.090 | 0.521 |
| Number of reflections | 43097 | |
| <I/σ(I)> | 22.6 | 2 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2M calcium acetate, 0.1M MES:NaCl, 20% (w/v) PEG8000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
