4R7H

Crystal structure of FMS KINASE domain with a small molecular inhibitor, PLX3397

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11587
Spacegroup name	P 43 21 2
Unit cell lengths	63.984, 63.984, 185.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.625 - 2.800
R-factor	0.2222
Rwork	0.221
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hw7
RMSD bond length	0.004
RMSD bond angle	0.727
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	60.471
High resolution limit [Å]	2.800
Number of reflections	10071

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	20% PEG8K, 0.2M MGCL AND 0.1M TRIS, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K