4R7A

Crystal Structure of RBBP4 bound to PHF6 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	50.630, 87.550, 95.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.830 - 1.850
R-factor	0.1779
Rwork	0.176
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gfc
RMSD bond length	0.006
RMSD bond angle	1.178
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.770	1.950
High resolution limit [Å]	1.850	1.850
Number of reflections	35775
Completeness [%]	96.2	96.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1M potassium thiocyanate,30% PEGMME2000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K