4R5Z
Crystal structure of Rv3772 encoded aminotransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-11 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 256.920, 77.556, 117.914 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.410 - 1.950 |
R-factor | 0.18012 |
Rwork | 0.179 |
R-free | 0.20262 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ffh |
RMSD bond length | 0.006 |
RMSD bond angle | 1.150 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 167040 | |
Completeness [%] | 97.0 | 93.6 |
Redundancy | 5.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1M succinic acid, 0.1M HEPES, 1% PEG 2000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |