4R2W
X-ray structure of uridine phosphorylase from Shewanella oneidensis MR-1 in complex with uridine at 1.6 A resolution
Replaces: 4HERExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.812 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.920, 96.480, 91.930 |
| Unit cell angles | 90.00, 120.01, 90.00 |
Refinement procedure
| Resolution | 19.490 - 1.600 |
| R-factor | 0.17805 |
| Rwork | 0.177 |
| R-free | 0.19188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rxy |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.928 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 79.620 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 171553 |
| Completeness [%] | 77.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.75M ammonium sulfate, 0.075M Bis-Tris, pH 5.5, 0.75% (w/v) PEG 3350, 25% (v/v) glycerol, VAPOR DIFFUSION, HANGING DROP , temperature 293.0K |
