4R28
MspJI Restriction Endonuclease in Complex with 27-mer Oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 88.544, 88.544, 511.971 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.032 - 3.055 |
| R-factor | 0.2301 |
| Rwork | 0.227 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f0q |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.564 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.032 | 3.160 |
| High resolution limit [Å] | 3.055 | 3.060 |
| Rmerge | 0.117 | 0.698 |
| Number of reflections | 42723 | |
| <I/σ(I)> | 10.8 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 289 | 12% polyethylene glycol MME 2000, 14% Tacsimate (Hamption Research), pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
