4R10
A conserved phosphorylation switch controls the interaction between cadherin and beta-catenin in vitro and in vivo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97945 |
Spacegroup name | P 43 |
Unit cell lengths | 84.720, 84.720, 136.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.469 - 2.300 |
R-factor | 0.1775 |
Rwork | 0.175 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.721 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.046 | 0.019 | 0.421 |
Number of reflections | 42695 | ||
<I/σ(I)> | 14.5 | ||
Completeness [%] | 99.6 | 99.5 | 99.3 |
Redundancy | 3.9 | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.2M sodium iodide, 17% PEG 3350, 0.1M Bis-Tris-Propane, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |