4R05
Crystal structure of the refolded DENV3 methyltransferase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 44.688, 48.386, 68.073 |
| 格子定数 [度] | 79.11, 78.75, 69.71 |
精密化法
| 残基 | 33.092 - 2.100 |
| R因子 | 0.2526 |
| Rwork | 0.250 |
| R-free | 0.30720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p8z |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.821 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 45.000 | 2.180 |
| 分解能 [Å] (高) | 2.100 | 2.100 |
| Rmerge_l_obs | 0.123 | 0.450 |
| 独立反射数 | 28779 | |
| <I/σ(I)> | 3.9 | 1.3 |
| 完全性 [%] | 94.9 | 92.7 |
| 冗長性 | 2.1 | 2.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 50 mM sodium citrate, pH 5.6, 24-30% PEG 4,000, 5% saturated ammonium sulfate, 10% glycerol, 0-20% DMSO, and 5 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
