4QZH
Mouse Tdt, F401A mutant, in complex with a DSB substrate, C-T base pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 56.309, 75.009, 125.611 |
Unit cell angles | 90.00, 97.64, 90.00 |
Refinement procedure
Resolution | 41.500 - 2.600 |
R-factor | 0.1977 |
Rwork | 0.196 |
R-free | 0.22140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i2a |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.051 | 0.655 |
Number of reflections | 16036 | |
<I/σ(I)> | 16.2 | 2.1 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |