4QZ8

Mouse Tdt in complex with a DSB substrate, C-G base pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.872600
Spacegroup name	C 1 2 1
Unit cell lengths	59.329, 74.259, 118.969
Unit cell angles	90.00, 97.98, 90.00

Refinement procedure

Resolution	31.410 - 2.700
R-factor	0.2054
Rwork	0.204
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i2a
RMSD bond length	0.010
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.700	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.060	0.640
Number of reflections	14082
<I/σ(I)>	14.4	2.3
Completeness [%]	99.3	99.8
Redundancy	4.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K